Olympus TIRF 4-Line SMT

Microscope

Inverted research microscope Olympus IX-81 (manual) equipped with

motorized filter cassette
motorized revolving nosepiece
motorized z-drive with remote focus handle
hardware autofocus (z drift compensation module)
piezo z-stage (NanoScanZ, NZ100) from Prior
- Range of motion: 100 µm
- Repeatability: 1 nm
- Accuracy: 0.5 % of travel
- fully software-controlled
motorized xy-stage IM 120x80 from Märzhäuser
- travel range: 120 x 80 mm
- repeatability: < 1µm
- resolution: 0.01 µm
100 W halogen lamp for transmission light with electronic shutter
Mid-long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55)
DIC imaging via:
- polarizer U-POT (added on top of condenser)
- Dedicated DIC prisms for 20x (IX2-MDIC20), 60x (IX2-MDIC60), and 100x (IX2-MDIC100) objectives
- DIC slider U-DICTS (below revolving noisepiece)
- DIC analyzer IX2-MDICT (located in filter turret)
Optional optovar lens: 1.6x
Microscope large incubator from Pecon:
- warm air incubation controlled with TempController 2000-2: RT - 60 °C, resolution: 0.01 °C
Small stage top incubator for controlled CO₂ concentration and 90 % humidity
- CO₂ is controlled via CO₂-Controller 2000: 0 - 20 Vol-%, resolution: 0.01 Vol-%
the complete setup is mounted on an active vibration isolation table (Science desk, Melles Griot)