Olympus TIRF 4-Line SMT
Filter
Clean-up filter of 4-Line TIRF condenser- 405 nm laser: bandpass, 370 - 410 nm (BrightLine HC 390/40)
- 488 nm laser: bandpass, 464 - 500 nm (BrightLine HC 482/18)
- 561 nm laser: bandpass, 547 - 575 nm (BrightLine HC 561/14)
- 640 nm laser: bandpass, 626 - 654 nm (BrightLine HC 640/14)
- Pos. 1: quadcolor (blue, green, orange, red)
- Beamsplitter: TIRF quadband polychroic mirror, 405 nm/488 nm/561 nm/640 nm (zt405/488/561/640rpc)
- Emitter: quadbandpass, 446 nm/523 nm/600 nm/677 nm (BrightLine HC 446/523/500/677)
- Pos. 2-5: none
- Pos. 6: IX2-MDICT (analyzer for DIC imaging)
Fast Emission Filter Wheel- Pos. 1: blue channel (DAPI, BFP): bandpass 423 - 468 nm, (BrightLine HC 445/45)
- Pos. 2: green channel (GFP): bandpass 500 - 550 nm, (BrightLine HC 525/50)
- Pos. 3: orange channel (TMR, mCherry): bandpass 582 - 619 nm, (BrightLine HC 600/37)
- Pos. 4: red channel (Cy5, Atto 655): bandpass 668 - 726 nm, (BrightLine HC 697/58)
- Pos. 5-8: empty
Quadview filter cube
- Dichroic beamsplitter
- 1: 480 nm, longpass (480dcxr)
- 2: 565 nm, longpass (565dcxr)
- 3: 640 nm, longpass (640dcxr)
- Emission filter
- Blue channel (DAPI, BFP): bandpass, 426 - 450 nm (BrightLine HC 438/24)
- Green channel (GFP): bandpass, 502 - 538 nm (BrightLine HC 520/35)
- Orange channel (TMR, mCherry): bandpass 582 - 619 nm, (BrightLine HC 600/37)
- Red channel (Cy5, Atto 655): bandpass, 665 - 705 nm (Brightline HC 685/40)