Olympus TIRF 4-Line STORM

Microscope

Inverted research microscope Olympus IX-83 equipped with

motorized 8-position filter cassette
motorized revolving nosepiece
motorized ultrasonic xy-stage (IX3-SSU, Olympus)
hardware autofocus (IX3-ZDC2, 830 nm version, Olympus)
white LED (Thorlabs) for transmission light with electronic shutter
long working distance condenser IX2-LWUCD (working distance 27 mm, NA 0.55) with DIC prisms (60x, 100x)
DIC imaging via:
- polarizer U-POT (added on top of condenser)
- Dedicated DIC prisms for 60x (IX2-MDIC60), and 100x (IX2-MDIC100) objectives
- motorized DIC slider (below revolving noisepiece)
- DIC analyzer IX3-DICTA (located in filter turret)
Microscope large incubator cellVivo from Pecon:
- warm air controlled with TempController 2000-2: RT - 60 °C, resolution: 0.01 °C
Small stage top incubator for controlled CO₂ as well as O₂ concentration and 90 % humidity
- CO₂ and O₂ is controlled via CO₂-O₂-Controller 2000: 0 - 20 Vol-%, resolution: 0.01 Vol-%