Lattice Light-Sheet Microscope

Sample Preparation

Sample support needs to be an 100 µm thick coverslip with 5 mm in diameter (CS-5R, Warner Instruments). These cover slips can be easily used for cell culture of adherent cells. Immobilization of non-adherent cells such as bacteria or yeast works well with poly-L-lysine or concanavalin A coatings. Bigger organisms such as zebrafish embryos are immobilized by low temperature melting agarose (<=1%). Nematodes like C. elegans or drosophila embryos can be fixed by embryo glue. The sample is mounted via a stainless steel sample holder with a metal clip fixing the cover slip (see picture). There is a very helpful blog from the Advanced Imaging Center (AIC) at Janelia Research Campus. Sample preparation for LLSM is described here in detail.