Light Microscopy
Overview
The integrated Bioimaging facility iBiOs is a core facility within the Center for Cellular Nanoanalytics (CellNanOs), an interdisciplinary Research Center at Osnabrück University. The iBiOs provides access to advanced fluorescence and electron microscopy techniques with highest spatiotemporal resolution. Next to state-of-the-art fluorescence imaging techniques, the iBiOs has specialized on live cell super-resolution microscopy based on single molecule imaging, structured illumination (SIM) and spectroscopy (FLIM, FCS). The iBiOs provides access to cutting-edge techniques such as lattice light-sheet microscopy and develops new nanoparticle based methods such as upconversion microscopy and second harmonic generation microscopy in close collaboration with different research groups at CellNanOs.
The EM unit provides a broad range of routine and advanced 2D and 3D EM and correlative light and electron microscopy (CLEM) workflows combined with customized image analysis. CLEM combines the advantages of fluorescence microscopy (FM) and EM to locate specific labeled events in its underlying ultrastructural context. Different CLEM approaches, including live-cell FM before EM or targeting sites within multi-cellular organisms for subsequent EM have been established and further developed. The newly established Structural Biology Division, led by Arne Möller, complements the existing technologies with the expertise in high-resolution cryo-EM applications, especially singl-particle analysis. State-of-the-art cryo-sample preparation pipelines (high-pressure and plunge freezing, cryo-microtomy) were recently established.
The cytometry unit offers expertise and support for the use of various flow cytometers of the department (three analysers and one sorter). Available techniques include high-speed cell sorting, single cell cloning, expression analysis of fluorescent proteins, DNA ploidy and cell cycle analysis as well as measurement of apoptosis. Two of the instruments are placed in BSL2 labs and can be used to sort infectious material.
As a DFG-funded core facility, the iBiOs also provides access to external users. Please, contact us to discuss whether your project can be conducted at our imaging systems.
OMERO: https://omero.cellnanos.uni-osnabrueck.de/webclient/login/
Electronic Lab Notebook: https://openbis.cellnanos.uni-osnabrueck.de/openbis/
Huygens Deconvolution: https://huygens.cellnanos.uni-osnabrueck.de/
The EM unit provides a broad range of routine and advanced 2D and 3D EM and correlative light and electron microscopy (CLEM) workflows combined with customized image analysis. CLEM combines the advantages of fluorescence microscopy (FM) and EM to locate specific labeled events in its underlying ultrastructural context. Different CLEM approaches, including live-cell FM before EM or targeting sites within multi-cellular organisms for subsequent EM have been established and further developed. The newly established Structural Biology Division, led by Arne Möller, complements the existing technologies with the expertise in high-resolution cryo-EM applications, especially singl-particle analysis. State-of-the-art cryo-sample preparation pipelines (high-pressure and plunge freezing, cryo-microtomy) were recently established.
The cytometry unit offers expertise and support for the use of various flow cytometers of the department (three analysers and one sorter). Available techniques include high-speed cell sorting, single cell cloning, expression analysis of fluorescent proteins, DNA ploidy and cell cycle analysis as well as measurement of apoptosis. Two of the instruments are placed in BSL2 labs and can be used to sort infectious material.
As a DFG-funded core facility, the iBiOs also provides access to external users. Please, contact us to discuss whether your project can be conducted at our imaging systems.
Direct Link To
iBiOs Booking: https://www.ibios.uni-osnabrueck.de/booked/WebOMERO: https://omero.cellnanos.uni-osnabrueck.de/webclient/login/
Electronic Lab Notebook: https://openbis.cellnanos.uni-osnabrueck.de/openbis/
Huygens Deconvolution: https://huygens.cellnanos.uni-osnabrueck.de/